Resources

We believe in open science and in sharing meaningful research outputs beyond traditional publications. Our lab is committed to making protocols, data analysis and datasets open and easily accessible for anyone to use, reproduce and build upon.

2025

pCas9-Duo gRNA verification

Abhinay Ramaprasad

[no publisher info]  ·  11 Jan 2025  ·  [no id info]

to screen for pCas9-Duo colonies positive for both guides.

Publication

shiftiko tool code

2024

Design strategy for DiCre-mediated inducible gene knockout in the malaria parasite

Anagha Rajesh Salvi, Abhinay Ramaprasad

protocols.io  ·  24 Dec 2024  ·  doi:10.17504/protocols.io.eq2ly68begx9/v1

This protocol describes a detailed strategy for inducibly knocking out a target gene in Plasmodium species using the Dimerisable Cre (DiCre) recombinase system, with a primary focus on Plasmodium falciparum.

dicre protocols

Single-step assembly of double guide plasmid (pCas9-Duo) for gene-editing in Plasmodium

Abhinay Ramaprasad

protocols.io  ·  11 Jun 2024  ·  doi:10.17504/protocols.io.n2bvjn9d5gk5/v1

This protocol describes a one-pot GoldenGate assembly of a new dual-guide targeting plasmid (pCas9-Duo) expressing two distinct guide RNAs in order to enhance the chances of a successful Cas9-mediated modification at a target region in Plasmodium falciparum.

Publication

shiftiko dicre protocols

Plasmid and gene loci maps related to SHIFTiKO

Abhinay Ramaprasad

Github  ·  21 Jan 2024  ·  [no id info]

Publication

shiftiko dicre plasmid-maps

2023

R notebook associated with the LCAT publication

Abhinay Ramaprasad

[no publisher info]  ·  29 May 2023  ·  [no id info]

useful for exploring data on LCAT (PF3D7_0629300)-null mutants.

Publication

dicre dataset code

R notebook associated with the GDPD publication

Abhinay Ramaprasad

[no publisher info]  ·  14 May 2023  ·  [no id info]

useful for exploring lipidomics data of GDPD (PF3D7_1406300)-null mutants.

Publication

dicre dataset code